Effect of expansion of Shine-Dalgarno sequence for overexpression of nicotinamide adenine dinucleotide oxidase gene from Deinococcus geothermalis in Escherichia coli
نویسندگان
چکیده
Thermostable enzymes have applications in biosensors and bioreactors. Overexpression of thermostable enzyme-encoding genes from thermophiles Escherichia coli is necessary to produce sufficient quantities such enzymes. Deinococcus gerthermalis a moderately thermophilic bacterium has Nicotinamide adenine dinucleotide (NADH) an important coenzyme for various dehydrogenases. NADH oxidase (Nox) catalyze the oxidation NADH. Therefore, Nox versatile enzyme that can be used combination with many types biosensors. This study reveals effect expansion SD sequence leader gene D. geothermalis overexpression E. coli, shows while 1- or 2-bases Shine-Dalgarno effective. should contributed expression coli.
منابع مشابه
Mode of nicotinamide adenine dinucleotide utilization by Escherichia coli.
Exogenous nicotinamide adenine dinucleotide is not utilized per se by Escherichia coli, but is converted to nicotinamide and thence to nicotinamide adenine dinucleotide via nicotinate.
متن کاملEvaluating Representations for the Shine-Dalgarno Site in Escherichia coli
Several methods for identifying individual motif instance by exhaustive evaluation of k-mers (k ≤ 10) are applied to the pooled Upstream Regions (USR) of all 4289 Escherichia coli ORFs. Instances of the Shine-Dalgarno (SD) site are readily identified using these methods. Using these motif instances as starting points, various motif representations and training methods, including several new alg...
متن کاملAn Allosteric Reduced Nicotinamide Adenine Dinucleotide Oxidase from Mycobacterium Tuberculosis.
The activation and inhibition of enzymes by compounds not directly involved in the enzyme-catalyzed reaction has been known for some time (1). The general importance of this effect as a control mechanism has not been recognized until recently; Illonod, Changeux, and Jacob (2) reviewed this type of metabolic control, brought about by what they have termed allosteric effecters. This paper present...
متن کاملSubstituted Nicotinamide Analogues of Nicotinamide Adenine Dinucleotide.
A number of nicotinamide adenine dinucleotide analogues have been prepared in which the purine, pyridine, and ribose moieties have been modified (2-12). These analogues have proven to be valuable in studies dealing with the site of binding of the pyridine coenzyme to dehydrogenases, in elucidating the mechanism of dehydrogenases and the configuration of the pyridme coenzymes, and as indicators ...
متن کاملSubstituted Nicotinamide Analogues of Nicotinamide Adenine Dinucleotide*
A number of nicotinamide adenine dinucleotide analogues have been prepared in which the purine, pyridine, and ribose moieties have been modified (2-12). These analogues have proven to be valuable in studies dealing with the site of binding of the pyridine coenzyme to dehydrogenases, in elucidating the mechanism of dehydrogenases and the configuration of the pyridme coenzymes, and as indicators ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Medical research archives
سال: 2023
ISSN: ['2375-1916', '2375-1924']
DOI: https://doi.org/10.18103/mra.v11i7.2.4172